S. Jergic

A bipartite interaction with the processivity clamp potentiates Pol IV-mediated TLS

This study shows that a unique secondary contact of Pol IV with the processivity clamp enhances its ability to perform translesion synthesis past strong replication blocks.

Single-molecule visualization of sequence-specific RNA binding by a designer PPR protein

This study investigates how designer PPR proteins bind to specific RNA sequences, showing they do not scan longer RNAs and instead rely on diffusion, with implications for their localization in organelles.

Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli

This study presents six high-resolution structures of the E. coli clamp loader complex (CLC) during the clamp loading process, revealing the molecular mechanism of clamp opening and DNA binding.

Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase

This study visualizes how the Escherichia coli Rep helicase rescues stalled replication forks by removing roadblocks, showing replication restart is the rate-limiting step.

The E. coli helicase does not use ATP during replication.

The paper shows that the E. coli DnaB helicase does not use ATP during DNA replication, with polymerase-driven nucleotide incorporation providing the energy instead.

Mechanism of transcription modulation by the transcription-repair coupling factor

This study investigates how the bacterial TRCF Mfd modulates transcription by converting elongation complexes into catalytically poised states and then irreversibly terminating transcription through ATP hydrolysis.

A biophysical and structural analysis of DNA binding by oligomeric hSSB1 (NABP2/OBFC2B)

This study investigates how oligomeric hSSB1 binds to single-stranded DNA, showing it interacts with both damaged and undamaged bases and binds longer DNA with higher affinity than its monomeric form.

Modulation of DNA polymerase IV activity by UmuD and RecA* observed by single-molecule time-lapse microscopy

The paper shows UmuD and RecA modulate DNA polymerase IV binding to DNA substrates, with UmuD inhibiting and RecA promoting association, particularly in DNA-damaged cells.

A gatekeeping function of the replicative polymerase controls pathway choice in the resolution of lesion-stalled replisomes

This study shows how the replicative polymerase's proofreading subunit controls lesion resolution pathways in bacteria by restricting access to TLS polymerases.

Recycling of single-stranded DNA-binding protein by the bacterial replisome.

This study visualizes how the E. coli replisome recruits and recycles single-stranded DNA-binding protein using two mechanisms, showing their physiological relevance.

A Primase-Induced Conformational Switch Controls the Stability of the Bacterial Replisome

This study identifies a primase-induced conformational switch that enhances the stability of the bacterial replisome by strengthening the interaction between the polymerase holoenzyme and the replicative helicase.

Nuclease dead Cas9 is a programmable roadblock for DNA replication

The paper uses a modified Cas9 protein to create a programmable roadblock for studying DNA replication fork arrest in vitro and in vivo.

Spatial and temporal organization of RecA in the Escherichia coli DNA-damage response

This study uses a fluorescent probe to track RecA dynamics in live E. coli, showing it is stored during normal metabolism and rapidly mobilizes to form SOS-signaling complexes upon DNA damage.