Assembly and translocation of a CRISPR-Cas primed acquisition complex
M. W. Brown, K. E. Dillard, Y. Xiao, A. E. Dolan, E. T. Hernandez, S. Dahlhauser, Y. Kim, L. R. Myler, E. Anslyn, A. Ke, I. Finkelstein
This study uses single-molecule techniques to characterize the type I-E CRISPR-Cas primed acquisition complex, revealing how Cascade, Cas3, and Cas1-Cas2 coordinate to recognize and acquire foreign DNA.
bioRxiv · Oct 26, 2017Biophysics