Molecular BiologyNucleic Acids ResearchPeer-reviewed
Gene-sized DNA insertion at genomic safe harbors in human cells using a site-directed transposase
James E Short, Lisa Sharek, Joshua F. Meckler…·Jesse B. Owens
This paper develops a site-directed transposase system for precise genomic insertion of gene-sized DNA without double-strand breaks, achieving on-target insertion with minimal off-target effects.
2 citations