J. E. Hill

Rapid and accurate taxonomic classification of cpn60 amplicon sequence variants

This study trains and tests a naive Bayesian classifier for cpn60 amplicon sequence variants, achieving high species-level classification accuracy in human microbiome datasets.

Transport and utilization of glycogen breakdown products by Gardnerella spp. from the human vaginal microbiome

This study identifies transporter systems in Gardnerella species that facilitate the uptake of glycogen breakdown products, revealing species-specific preferences for longer chain malto-oligosaccharides.

Glycogen degrading activities of catalytic domains of α-amylase and α-amylase-pullulanase enzymes conserved in Gardnerella spp. from the vaginal microbiome

This study identifies and characterizes glycogen-degrading enzymes in Gardnerella spp. that break down glycogen into maltose, maltotriose, and maltotetraose, contributing to nutrient competition in the vaginal microbiome.

Reduced metabolic activity in established Gardnerella spp. biofilms contributes to protection from the bactericidal effects of metronidazole

This study shows that Gardnerella spp. biofilms have reduced metabolic activity when exposed to metronidazole, which may explain their resistance to the antibiotic.

Characterization of an α-glucosidase enzyme conserved in Gardnerella spp. isolated from the human vaginal microbiome

This study identifies and characterizes a conserved α-glucosidase in Gardnerella spp. that hydrolyzes maltooligosaccharides but not glycogen, suggesting a role in glycogen metabolism in the vaginal microbiome.

cpn60 barcode sequences accurately identify newly defined genera within the Lactobacillaceae

The study updates a reference database and validates the cpn60 barcode's accuracy for identifying lactobacilli species under a new taxonomic framework.

Population density affects the outcome of competition in co-cultures of Gardnerella species isolated from the human vaginal microbiome

The paper shows that population density affects competition outcomes between Gardnerella subgroup D and C isolates from the human vaginal microbiome.

Slipped strand mispairing in the gene encoding sialidase NanH3 in Gardnerella spp.

The paper identifies slipped-strand mispairing in the nanH3 gene of Gardnerella spp., leading to phase variation in sialidase expression and potential immune evasion or biofilm formation.

A generalist lifestyle allows rare Gardnerella spp. to persist at low levels in the vaginal microbiome

The paper shows that subgroup D of Gardnerella spp. persists in the vaginal microbiome due to a generalist lifestyle and negative-frequency dependent selection.

Assessment of metagenomic sequencing and qPCR for detection of influenza D virus in bovine respiratory tract samples

This study compares metagenomic sequencing and qPCR for detecting influenza D virus in bovine samples, finding varying agreement rates between methods.

Competition among Gardnerella subgroups from the human vaginal microbiome

The paper investigates interactions among Gardnerella subgroups in vitro, finding competition reduces growth rates of subgroups A, B, and C while increasing subgroup D's growth.

Resolution and co-occurrence patterns of Gardnerella leopoldii, Gardnerella swidsinskii, Gardnerella piotii and Gardnerella vaginalis within the vaginal microbiome

The paper identifies co-occurrence patterns of Gardnerella species in vaginal microbiomes and shows that cpn60 sequencing resolves them better than 16S rRNA.

Silencing cryptic specialized metabolism in Streptomyces by the nucleoid-associated protein Lsr2

This study shows that Lsr2 represses specialized metabolic gene clusters in Streptomyces, revealing its role in silencing cryptic metabolism and controlling the production of secondary metabolites.