X. Zhang

TSNR: Protein-Specific Signals with Broad Molecular Context for Spatial Protein Prediction

The paper introduces TSNR, a method for spatial protein prediction that uses protein-specific signals and broad molecular context, achieving high correlation on a benchmark.

Heparan sulfate promotes autoactivation of pro-Cathepsin K by destabilizing the propeptide-catalytic domain interaction

This study shows heparan sulfate promotes pro-CtsK autoprocessing by disrupting an electrostatic interaction between the propeptide and catalytic domain.

Mitoregulin supports mitochondrial membrane integrity and protects against cardiac ischemia-reperfusion injury

This study shows that Mitoregulin (Mtln) protects mitochondrial membrane integrity and reduces cardiac ischemia-reperfusion injury by stabilizing cardiolipin and preventing membrane damage.

Heparan sulfate selectively inhibits the collagenase activity of cathepsin K

This study shows heparan sulfate inhibits cathepsin K collagenase activity while preserving peptidase activity, revealing HS as a potential therapeutic target for bone resorption diseases.

A New Large-Scale Extracellular Vesicle Production Strategy for Biomedical Drug Development

This paper introduces a novel large-scale EV production strategy with upstream fed-batch suspension cell culture and downstream chromatographic purification for clinical applications.

Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections

This paper introduces an optimized smFISH protocol for plant tissue cryosections, enabling precise quantification of mRNA and validation of scRNA-seq data.

All-trans retinoic acid induces durable tumor immunity in IDH-mutant gliomas by rescuing transcriptional repression of the CRBP1-retinoic acid axis

This study shows that all-trans retinoic acid (ATRA) improves immune response in IDH-mutant gliomas by restoring the RA pathway, leading to tumor regression in mouse models and a clinical trial for human patients.

MicroRNAs and PFAS: A Pilot Study in Blood Collected from Firefighters

This study examines PFAS exposure in firefighters through miRNA sequencing of plasma exosomes and compares results to general population data, finding no significant overall PFAS difference but miRNA expression heterogeneity.

SwinCell: a transformer-based framework for dense 3D cellular segmentation

SwinCell is a 3D transformer-based framework that improves segmentation of dense cellular images by balancing local feature recognition and contextual understanding.

Onco-Circuit Addiction and Onco-Nutrient mTORC1 Signaling Vulnerability in a Model of Aggressive T Cell Malignancy

The paper shows that mTORC1 signaling hypersensitivity to leucine drives an onco-circuit in T cell malignancy, and a low leucine diet is therapeutic without impairing T cell immunity.

Kidney toxicology of a novel compound Lithium Bis(trifluoromethanesulfonyl)imide (LiTFSI, ie. HQ-115) used in energy applications: an Epigenetic evaluation

This study evaluates the kidney toxicology of LiTFSI (HQ-115) in mice, finding biochemical changes, renal injuries, and epigenetic alterations linked to inflammation and cancer pathways.

Proliferative cell targeting and epithelial cell turnover fuels hepatitis E virus replication in human intestinal enteroids

This study establishes an HEV-HIE model showing HEV replication in intestinal epithelial cells, with proliferative cells being the main targets, suggesting the gut as an HEV reservoir.

Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension

This study shows that increased TRPV4 channel expression in endothelial cells both enhances and impairs vascular function in hypertension, leading to altered Ca2+ signaling and vascular reactivity.

Differential seasonal effects of ephemeral legumes in response to moisture-nitrogen coupling in the deserts of northwestern China

This study examines how moisture and nitrogen affect two desert legumes, showing growth and chlorophyll differences under varying treatments, with moisture-nitrogen coupling influencing physiological responses.

Cyborg islets: implanted flexible electronics reveal principles of human islet electrical maturation

The paper uses flexible electronics to study electrical maturation of human pancreatic islets over time, identifying key electrical states and their role in hormone secretion.

Engineered Migrasomes: Harnessing Core Migrasome Machinery and Hypotonic Shock to Develop a Robust and Thermally Stable Vaccine Platform

This study engineers migrasome-like vesicles (eMigrasomes) using hypotonic shock to create a stable, thermally robust vaccine platform that elicits strong immune responses.

The Kv2.2 channel mediates the inhibition of Prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells

This study shows PGE2 inhibits glucose-stimulated insulin secretion in β-cells via the EP2/4-Kv2.2 pathway by modulating Kv2.2 channels through PKA.

Cytokine and Chemokine Receptor Profiles in Adipose Tissue Vasculature Unravel Endothelial Cell Responses in HIV

This study examines cytokine and chemokine receptor expression in adipose tissue endothelial cells of HIV-positive individuals, identifying receptor differences and their potential role in vascular dysfunction.

MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

This study identifies MYB36 subclade transcription factors as regulators of cork differentiation by promoting suberin deposition and repressing cork cambium proliferation.

Protocatechuic acid induces endogenous oxidative stress in CR-hvKP by regulating the EMP-PPP pathway

This study shows that protocatechuic acid (PCA) enhances antibiotic efficacy against carbapenem-resistant hypervirulent Klebsiella pneumoniae by inducing oxidative stress through regulation of the EMP-PPP pathway.

Subtomogram averaging of helical filaments in cryo-electron tomography

The paper describes a protocol for subtomogram averaging to generate high-resolution density maps of helical filaments using cryo-electron tomography, applied to mumps virus nucleocapsids and in-cell forms.

Enhanced Ca2+-Driven Arrhythmias in Female Patients with Atrial Fibrillation: Insights from Computational Modeling

This study uses computational models to show female atrial cardiomyocytes in AF have higher Ca2+-driven arrhythmia risk than males, with specific interventions identified to address these differences.

Multi-COBRA hemagglutinin formulated with cGAMP microparticles elicit protective immune responses against influenza viruses

This study evaluates COBRA vaccines formulated with cGAMP microparticles in mice, showing enhanced antibody responses and protection against influenza viruses.

DACIT: Device for Axon - Cancer cell Interaction Testing in 2D and 3D

DACIT enables in vitro study of axon-cancer cell interactions in 2D and 3D by separating neuronal soma from axons and cancer cells into distinct compartments.

Gel-Assisted Proteome Position Integral Shift (GAPPIS) Assay Returns Molecular Weight to Shotgun Proteomics and Identifies Novel Caspase 3 Substrates

The paper introduces GAPPIS, a method to recover molecular weight information from shotgun proteomics, identifying novel caspase 3 substrates in apoptotic cells.

Do light eaters live shorter lives? The case of ultralight Caenorhabditis elegans

This study shows that ultralight Caenorhabditis elegans, grown on isotopically depleted bacteria, have shorter lifespans despite faster growth and maturation.

Photo-Uncaging Triggers on Self-Blinking to Control Single-Molecule Fluorescence Kinetics for Super-Resolution Imaging

The paper develops a new fluorophore, NOSR, that enables controlled single-molecule blinking for improved super-resolution imaging of microtubules and mitochondrial outer membranes.

A Specialized Centrosome-Proteasome Axis Mediates Proteostasis and Influences Cardiac Stress through Txlnb

This study identifies Txlnb as a centrosome-adapter protein that mediates proteostasis in cardiomyocytes, linking centrosomes to the ubiquitin-proteasome system and influencing cardiac stress responses.

Unveiling Tryptophan Dynamics and Functions Across Model Organisms via Quantitative Imaging

This study uses GRIT to measure tryptophan dynamics across species and scales, revealing links between tryptophan metabolism, inflammation, and behavior.

In vivo affinity maturation of the HIV-1 Env-binding domain of CD4

This study demonstrates that non-antibody protein biologics can undergo affinity maturation in vivo, leading to improved binding affinity and neutralization potency against HIV-1.