This paper introduces a 4Pi MINFLUX technique that achieves high spatio-temporal localization precision for single fluorophores, revealing nanoscale diffusion heterogeneities in solutions.

bioRxiv · Oct 30, 2023Biophysics

MINFLUX dissects the unimpeded walking of kinesin-1

J. O. Wolff, L. Scheiderer, T. Engelhardt, J. Engelhardt, J. Matthias, S. W. Hell

This study uses MINFLUX microscopy to analyze kinesin-1 movement on microtubules, revealing structural changes and ATP dynamics during its stepping process.

bioRxiv · Jul 25, 2022Biophysics

Evolution of DNA methylation across Ecdysozoa

J. Engelhardt, O. Scheer, P. F. Stadler, S. J. Prohaska

The paper investigates DNA methylation evolution in Ecdysozoa, identifying multiple independent losses and predicting new instances of DNA methylation in previously unexamined phyla.

bioRxiv · Jul 15, 2021Evolutionary Biology

Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence

B. T. Werner, A. Koch, E. Secic, J. Engelhardt, L. Jelonek, J. Steinbrenner, K.-H. Kogel

This study shows that Fusarium graminearum uses DCL-dependent sRNAs to suppress host immune genes, enhancing its virulence.

bioRxiv · May 17, 2021Microbiology

Ecological plant epigenetics: Evidence from model and non-model species, and the way forward

C. L. Richards, C. Alonso, C. Becker, O. Bossdorf, E. Bucher, M. Colome-Tatche, W. Durka, J. Engelhardt, B. Gaspar, A. Gogol-Doring, I. Grosse, T. P. van Gurp, K. Heer, I. Kronholm, C. Lampei, , K. J. F. Verhoeven

The paper reviews epigenetic contributions to plant ecology and proposes integrating model-species research with non-model species studies to better understand ecological processes.

bioRxiv · Apr 25, 2017Ecology