S. W. Hell

MINFLUX fluorescence nanoscopy in biological tissue

This study demonstrates MINFLUX fluorescence nanoscopy's ability to achieve sub-10 nm resolution for protein localization in thick mouse brain tissue sections.

MINFLUX Reveals Dynein Stepping in Live Neurons

This study uses MINFLUX to track dynein movement in live neurons, revealing 8 nm steps and rapid direction reversals, suggesting a single ATP-dependent stepping mechanism.

Diffraction minima resolve point scatterers at tiny fractions (1/80) of the wavelength

The paper demonstrates that using diffraction minima allows resolving point scatterers at distances as small as 1/80 of the wavelength, improving measurement precision with closer scatterers and higher density.

Uncovering kinesin dynamics in neurites with MINFLUX

This study uses MINFLUX to analyze kinesin-1 dynamics in neurites, identifying ATP-binding states and microtubule switching behavior.

4Pi MINFLUX arrangement maximizes spatio-temporal localization precision of fluorescence emitter

This paper introduces a 4Pi MINFLUX technique that achieves high spatio-temporal localization precision for single fluorophores, revealing nanoscale diffusion heterogeneities in solutions.

MINSTED tracking of single biomolecules

The paper introduces MINSTED localization, a method for tracking labeled biomolecules with nanometer and millisecond precision using STED microscopy.

Direct optical measurement of intra-molecular distances down to the Angstrom scale

This paper demonstrates that MINFLUX fluorescence nanoscopy can measure intra-molecular distances down to 1 Angstrom with high precision, validated using known protein distances and subunit orientations.

Bioorthogonal caging-group-free photoactivatable probes for minimal-linkage-error nanoscopy

The paper introduces photoactivatable dyes for super-resolution microscopy, demonstrating their use in imaging vimentin filaments with high precision.

Photoactivatable large Stokes shift fluorophores for multicolor nanoscopy

The paper designs new photoactivatable fluorophores with large Stokes shifts for multicolor super-resolution microscopy in live cells.

Spontaneously blinking fluorophores for accelerated MINFLUX nanoscopy

The paper develops fluorophores with short on-times for MINFLUX nanoscopy, achieving faster localization by up to 30-fold.

MINFLUX dissects the unimpeded walking of kinesin-1

This study uses MINFLUX microscopy to analyze kinesin-1 movement on microtubules, revealing structural changes and ATP dynamics during its stepping process.

Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited

The paper re-analyzes MINFLUX 3D imaging data to confirm its high localization precision and discuss its implications for structural biology.

MINSTED nanoscopy enters the Angstrom localization range

This paper introduces MINSTED nanoscopy, achieving angstrom-scale localization of fluorophores with high precision using a STED microscope.

A general design of caging-group free photoactivatable fluorophores for live-cell nanoscopy

This paper introduces a method to create caging-group free photoactivatable fluorophores for live-cell nanoscopy, demonstrating their utility in multiple superresolution techniques.

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models

This paper develops a 4Pi-STORM microscope with dynamic spline PSF models to achieve high 3D resolution and accuracy in single molecule localization microscopy.

MINSTED fluorescence localization and nanoscopy

The paper introduces MINSTED, a STED-based super-resolution microscopy method that achieves high localization precision with reduced fluorophore bleaching, and demonstrates its application in imaging Mic60 proteins in human cells.

Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system

This paper demonstrates autonomous bioluminescence imaging of mammalian cells using the bacterial bioluminescence system, achieving luminescence comparable to firefly luciferase.

MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution in (living) cells

The paper demonstrates MINFLUX nanoscopy achieves 1-3 nanometer 3D-resolution in living cells using a doughnut-shaped excitation beam and iterative localization.