Pharmacology & ToxicologybioRxiv
DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery
N. J. Kapolka, G. J. Taghon, J. B. Rowe…·D. G. Isom
DCyFIR enables high-throughput CRISPR-based profiling of GPCRs and ligand discovery by integrating receptors into yeast genomes and analyzing ligand specificity across multiple strains.
DOI 10.1073/pnas.2000430117