J. Goedhart

The paper introduces studentsourcing, a method to collect, aggregate, and reuse data from a cell biology course, demonstrating its utility for both teaching and scientific research.

The paper shows that cellular networks can approach predictive information bounds for specific signal types but face trade-offs between information gain and cost, with implications for bacterial chemotaxis.

The paper introduces a method to recruit proteins to GFP-tagged targets using an antiGFP nanobody fused to the iLID system, enabling light-dependent localization without modifying the iLID.

This paper develops Opto-RhoGEFs, an optogenetic tool to control Rho GTPase activity in endothelial cells, revealing a VE-cadherin-independent mechanism for regulating vascular barrier strength.

The paper characterizes and optimizes relocation-based biosensors for Rac and Cdc42, improving their specificity and efficiency for cell-based applications.

This study identifies ARHGAP17 as a regulator of Cdc42 activity at invadopodia, showing it controls spatiotemporal Cdc42 activity during invadopodia formation and disassembly.

The paper identifies endothelial transmigration hotspots that limit vascular leakage by heterogeneous ICAM-1 expression, showing that ICAM-1 distribution determines hotspot location and function.

The paper investigates cell-to-cell heterogeneity in ERK and Akt activation by GPCRs, showing distinct dynamics depending on the activated G proteins.

This paper introduces a new calcium biosensor based on fluorescence lifetime imaging, which provides accurate and pH-insensitive quantification of intracellular calcium concentrations.

The paper provides methods to process and analyze timelapse imaging data for quantitative understanding of biological dynamics.

The paper improves RhoA localization-based biosensors to better detect endogenous RhoA activity in various cellular processes.

The paper identifies endothelial junctional membrane protrusions as preferred sites for neutrophil transmigration during inflammation and shows they depend on Rac1 activity.

The paper introduces a web app for transparently displaying and comparing continuous data from discrete conditions using advanced visualization techniques.

The paper develops a FRET biosensor, yEPAC, to measure cAMP signaling in budding yeast and finds that cAMP peaks vary with nutrient transitions and follow Weber's law.

PlotTwist is a web app that simplifies time-series data visualization and annotation without coding, offering normalization, multiple display formats, and annotation tools.

PlotsOfDifferences is a web tool that calculates effect sizes and their compatibility intervals for unpaired data using bootstrapping, providing graphical and tabular outputs with optional p-values.

The paper quantifies Cdc42 activation by endothelial GEFs in primary human cells, identifying FGD1 as the most selective activator.

PlotsOfData is a web app that visualizes raw data with statistical summaries using ggplot2, offering flexibility in data display and interpretation.

The paper develops a new fluorescent protein variant, sfTq2ox, optimized for the bacterial periplasm to enable efficient FRET for studying cell division antibiotic targets.

The paper develops a FRET-based biosensor to measure Gα13 activation in single cells and demonstrates its use in detecting receptor-induced responses.

The paper evaluates fluorescent proteins as FRET acceptors for mTurquoise2, identifying mNeonGreen as the most efficient and photostable pair.

This study shows that membrane association of TGAT is critical for its activity, but palmitoylation is not required for membrane localization or RhoA activation.