J. D. van Buul

This paper develops Opto-RhoGEFs, an optogenetic tool to control Rho GTPase activity in endothelial cells, revealing a VE-cadherin-independent mechanism for regulating vascular barrier strength.

The paper identifies endothelial transmigration hotspots that limit vascular leakage by heterogeneous ICAM-1 expression, showing that ICAM-1 distribution determines hotspot location and function.

This study uses a blood-vessel-on-a-chip model to analyze how T-cells and neutrophils migrate differently in a tissue matrix after transendothelial migration.

This paper introduces a new calcium biosensor based on fluorescence lifetime imaging, which provides accurate and pH-insensitive quantification of intracellular calcium concentrations.

The paper improves RhoA localization-based biosensors to better detect endogenous RhoA activity in various cellular processes.

The paper identifies endothelial junctional membrane protrusions as preferred sites for neutrophil transmigration during inflammation and shows they depend on Rac1 activity.

This study identifies DLC1 as a direct target of YAP/TAZ that regulates endothelial dynamics and sprouting angiogenesis.