RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds
B. Fu, E. E. Brock, R. Andrews, J. C. Breiter, R. Tian, C. E. Toomey, J. Lachica, T. Lashley, M. Ryten, N. W. Wood, M. Vendruscolo, S. Gandhi, L. E. Weiss, J. S. Beckwith, S. F. Lee
RASP detects fluorescent puncta in complex cellular backgrounds with high precision and speed, outperforming existing methods by using image gradients to separate objects from noise.
bioRxiv · Dec 18, 2023Biophysics