M. Vendruscolo

AlphaFold Prediction of Structural Ensembles of Disordered Proteins

This paper shows that AlphaFold can predict structural ensembles for disordered proteins by using inter-residue distances.

The αC-β4 loop controls the allosteric cooperativity between nucleotide and substrate in the catalytic subunit of protein kinase A

This study identifies the αC-β4 loop as a key regulator of allosteric cooperativity between nucleotide and substrate in PKA-C through simulations and mutagenesis experiments.

Imaging Abeta aggregation by liquid-phase transmission electron microscopy

This study uses liquid-phase transmission electron microscopy to visualize Aβ aggregation dynamics and intermediate oligomers in solution.

The Alzheimer's Amyloid beta peptide forms biomolecular condensates that trigger amyloid aggregation

This paper shows that Alzheimer's amyloid-beta peptide forms biomolecular condensates on lipid bilayers, accelerating amyloid aggregation and revealing a new early step in the disease mechanism.

Transient interactions between the fuzzy coat and the cross-β core of brain-derived Aβ42 filaments

This study uses MEMMI to analyze the transient interactions between the fuzzy coat and cross-β core of Aβ42 filaments, revealing differences in solubility between familial and sporadic AD forms.

Aggregation of the Amyloid-beta Peptide (Abeta40) within Condensates Generated through Liquid-Liquid Phase Separation

This study shows that A{beta}40 can undergo liquid-liquid phase separation and that this process enhances amyloid fibril formation in Alzheimer's disease.

Nanoscale profiling of evolving intermolecular interactions in ageing FUS condensates

This study characterizes the aging behavior of FUS condensates using microfluidic technology and surface-based techniques, revealing phase transitions and structural changes associated with material properties.

RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds

RASP detects fluorescent puncta in complex cellular backgrounds with high precision and speed, outperforming existing methods by using image gradients to separate objects from noise.

CryoENsemble - a Bayesian approach for reweighting biomolecular structural ensembles using heterogeneous cryo-EM maps

CryoENsemble improves conformational ensembles from molecular dynamics simulations to better match cryo-EM data and extract dynamics information.

Multiplexed Digital Characterisation of Misfolded Protein Oligomers via Solid-State Nanopores

This paper introduces a single-molecule method using solid-state nanopores and DNA barcoding to detect and characterize misfolded protein oligomers, demonstrating its application to α-synuclein in Parkinson's disease.

Targeted protein editing with an antibody-based system

This paper introduces an antibody-based system for post-translational modification of endogenous proteins in complex environments, demonstrating its use on green fluorescent protein and beta-2 microglobulin.

α-Synuclein oligomers form by secondary nucleation

This study shows α-synuclein oligomers form via secondary nucleation on fibril surfaces, not from monomers, and this process is enhanced by agitation under physiological conditions.

Maturation-dependent changes in the structure and seeding capacity of Aβ42 amyloid fibrils

This study investigates how Aβ42 amyloid fibrils change over time, showing increased length, thickness, and cross-β sheet content, which affects their ability to seed new aggregates.

A scale-invariant log-normal droplet size distribution below the critical concentration for protein phase separation

The paper investigates droplet size distributions in protein phase separation and finds they follow a scale-invariant log-normal function, suggesting universal behavior and a method to estimate critical concentration.

ANXA11 biomolecular condensates facilitate protein-lipid phase coupling on lysosomal membranes

This study shows ANXA11 biomolecular condensates couple protein and lipid phase states on lysosomal membranes through interactions with ALG2 and CALC.

Sequence-based prediction of the solubility of peptides containing non-natural amino acids

The paper presents CamSol-PTM, a computational method to predict solubility of peptides with non-natural amino acids, validated through experimental testing of 30 variants.

A Kinetic Map of the Influence of Biomimetic Lipid Membrane Models on Aβ42 Aggregation

This study investigates how different lipid membrane models affect Aβ42 aggregation kinetics in vitro, showing inhibition by ER and Golgi membranes.

Structure-based discovery of small molecule inhibitors of the autocatalytic proliferation of alpha-synuclein aggregates

This study identifies small molecules that inhibit alpha-synuclein aggregate proliferation using structure-based and kinetic assays.

Multi-dimensional protein solubility optimization with an ultra-high-throughput microfluidic platform

This paper introduces a high-throughput microfluidic platform for protein solubility screening, enabling rapid and efficient optimization of formulation additives and mutations.

Enhanced surface nano-analytics of transient biomolecular processes

This paper develops a lab-on-a-chip method for analyzing biomolecular processes with high sensitivity and single molecule resolution in physiological conditions.

Determination of the structure and dynamics of the fuzzy coat of an amyloid fibril of IAPP using cryo-electron microscopy

The paper develops MEMMI, a cryo-EM method that determines structure and dynamics of large systems, and applies it to study the fuzzy coat of an IAPP amyloid fibril.

Sequence-based pH-dependent prediction of protein solubility using CamSol

The paper extends the CamSol method to predict protein solubility at different pH levels and validates it on antibodies and other proteins.

A Rapid in vivo Pipeline to Identify Small Molecule Inhibitors of Amyloid Aggregation

This paper presents an in vivo pipeline to identify small molecule inhibitors of amyloid aggregation by screening compounds that suppress crystal formation in C. elegans.

Discovery of Potent Inhibitors of α-Synuclein Aggregation Using Structure-Based Iterative Learning

This paper uses structure-based machine learning to identify potent small molecule inhibitors of α-synuclein aggregation, achieving compounds two orders of magnitude more potent than previous reports.

A small molecule stabilises the disordered native state of the Alzheimer's Aβ peptide

This study identifies a small molecule that stabilizes the disordered native state of the Alzheimer's Aβ42 peptide through dynamic binding involving π-stacking and entropic effects.

Vitamin A and vitamin E metabolites comodulate amyloid-β aggregation

This study identifies retinoic acid and tocopherol as vitamin metabolites that modulate amyloid-β aggregation in vitro and in a C. elegans model, with combined effects that may cancel each other.

Spontaneous nucleation and fast aggregate-dependent proliferation of α-synuclein aggregates within liquid condensates at physiological pH

Amyloidogenic proteins in the SARS-CoV and SARS-CoV-2 proteomes

This study investigates protein aggregation in SARS-CoV and SARS-CoV-2 proteomes, finding that several SARS-CoV-2 proteins form cytotoxic aggregates via a nucleation-dependent mechanism.

Environmental Dependence of the Structure of the C-terminal Domain of the SARS-CoV-2 Envelope Protein

This paper investigates how environmental factors affect the conformation of the C-terminal domain of the SARS-CoV-2 envelope protein, showing it transitions between disordered, membrane-bound, and amyloid states.

Sequence determinants of the aggregation of proteins within condensates generated by liquid-liquid phase separation

The paper identifies sequence determinants of protein aggregation within condensates formed by liquid-liquid phase separation and proposes a formula to detect aggregation-promoting mutations.