BiochemistrybioRxiv
A second specificity-determining loop in Class A sortases: Biochemical characterization of natural sequence variation in chimeric SrtA enzymes
I. M. Piper, S. A. Struyvenberg, J. D. Valgardson…·J. F. Amacher
The paper identifies a conserved loop in Class A sortases that influences substrate specificity and demonstrates how engineered chimeric enzymes can expand substrate tolerance.
DOI 10.1016/j.jbc.2021.100981