J. Ordon

Simultaneous tracking of near-isogenic bacterial strains in synthetic Arabidopsis microbiota by chromosomally-integrated barcodes

This paper introduces MoBacTags to track near-isogenic bacterial strains in synthetic Arabidopsis microbiota, revealing distinct roles of pqqF and cyoB in community establishment.

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

This study shows that using two pairs of guide RNAs with SpCas9 increases chromosomal deletion frequency in Arabidopsis, and TREX2 co-expression enhances deletion size without increasing deletion frequency.

Disentangling cause and consequence: Genetic dissection of the DANGEROUS MIX2 risk locus, and activation of the DM2h NLR in autoimmunity

The paper identifies DM2h as the gene responsible for autoimmunity in Arabidopsis thaliana and shows that EDS1-YFPNLS activates the DM2h NLR independently of its normal immune regulatory function.

Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants

The paper develops a toolkit for high-efficiency multiplex genome editing in plants, generating 8x Nicotiana benthamiana and 12x Arabidopsis mutants in a single generation.

An EDS1-SAG101 complex functions in TNL-mediated immunity in Solanaceae

The paper compares EDS1 complex functions in TNL-mediated immunity across Solanaceae species, finding that EDS1-SAG101 complexes are required in Nicotiana benthamiana, while EDS1-PAD4 complexes are functional in Arabidopsis.

Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation

The paper improves Cas9 expression systems in Arabidopsis to efficiently isolate complex mutant alleles in a single generation.

Peripheral infrastructure vectors and an extended set of plant parts for the modular cloning system

The paper introduces 80 new phytobricks and peripheral infrastructure for the Modular Cloning system to enhance its versatility in plant synthetic biology.