J. Stuttmann

Beyond defense: Glucosinolate structural diversity shapes recruitment of a metabolic network of leaf-associated bacteria

The paper shows that structural variations in glucosinolates affect bacterial community recruitment in Arabidopsis thaliana, with allyl-GLS enriching Burkholderiales bacteria.

Receptor-like cytoplasmic kinases belonging to different subfamilies mediate immune responses downstream of the Cf-4 resistance protein in Nicotiana benthamiana

This study identifies RLCK-VII subfamily members as essential for ROS production and signaling downstream of the Cf-4 resistance protein in N. benthamiana.

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

This study shows that using two pairs of guide RNAs with SpCas9 increases chromosomal deletion frequency in Arabidopsis, and TREX2 co-expression enhances deletion size without increasing deletion frequency.

Thylakoid grana stacking revealed by multiplex genome editing of LHCII encoding genes

This study investigates the molecular basis of thylakoid grana stacking in land plants by analyzing the role of LHCII encoding genes through multiplex genome editing.

Differential requirement for the EDS1 catalytic triad in A. thaliana and N. benthamiana

The paper investigates the functional differences of EDS1 catalytic triad in Arabidopsis and Nicotiana benthamiana, finding lineage-specific roles in TNL immunity and PRR signaling.

XopQ induced stromule formation in Nicotiana benthamiana is causally linked to ETI signaling and depends on ADR1 and NRG1

The paper investigates whether XopQ-induced stromule formation is linked to ETI signaling or programmed cell death in N. benthamiana and finds it is connected to ETI signaling but not cell death.

Membrane Contact Sites Between Chloroplasts and Pathogen Interface Underpin Plant Focal Immune Responses

This study identifies chloroplast-extrahaustorial membrane contact sites that regulate callose deposition during plant immune responses to pathogens.

Disentangling cause and consequence: Genetic dissection of the DANGEROUS MIX2 risk locus, and activation of the DM2h NLR in autoimmunity

The paper identifies DM2h as the gene responsible for autoimmunity in Arabidopsis thaliana and shows that EDS1-YFPNLS activates the DM2h NLR independently of its normal immune regulatory function.

Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants

The paper develops a toolkit for high-efficiency multiplex genome editing in plants, generating 8x Nicotiana benthamiana and 12x Arabidopsis mutants in a single generation.

An EDS1-SAG101 complex functions in TNL-mediated immunity in Solanaceae

The paper compares EDS1 complex functions in TNL-mediated immunity across Solanaceae species, finding that EDS1-SAG101 complexes are required in Nicotiana benthamiana, while EDS1-PAD4 complexes are functional in Arabidopsis.

A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors

The paper identifies a coevolved EDS1-SAG101-NRG1 module essential for TIR-domain immune receptor-mediated cell death signaling in plants.

Determining targeting specificity of nuclear-encoded organelle proteins with the self-assembling split fluorescent protein toolkit

The paper improves the sasplit-FP method to detect protein targeting specificity and develops a toolkit for high-throughput analysis of plastid and mitochondrial targeting in plants.

Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation

The paper improves Cas9 expression systems in Arabidopsis to efficiently isolate complex mutant alleles in a single generation.

Peripheral infrastructure vectors and an extended set of plant parts for the modular cloning system

The paper introduces 80 new phytobricks and peripheral infrastructure for the Modular Cloning system to enhance its versatility in plant synthetic biology.