GenomicsbioRxivPreprint
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Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore
John R. Tyson, Phillip L. James, David Stoddart…·Joshua Quick
V3 primer pools with 22 extra alternates improve coverage; revised library preps raise double-barcode demultiplexing from 42.15% to 74.5%, cut misassignment 35x and bring reagent cost near £10 per sample.