Optimized qRT-PCR approach for the detection of intra- and extracellular SARS-CoV-2 RNAs
T. Toptan, S. Hoehl, S. Westhaus, D. Bojkova, A. Berger, B. Rotter, K. Hoffmeier, S. Ciesek, M. Widera
This study presents an optimized qRT-PCR method for detecting SARS-CoV-2 RNA using a low-cost SYBR green approach, showing improved detection limits and simplicity compared to multi-step assays.
bioRxiv · Apr 20, 2020Microbiology