Bioluminescence-Based Determination of Cytosolic Accumulation of Antibiotics in Escherichia coli
R. Dash, K. Holsinger, M. D. Chordia, M. Sharifian Gh., M. Pires
This paper introduces a luciferase-based assay to measure antibiotic permeability into the cytosol of Gram-negative bacteria and demonstrates its ability to track intracellular accumulation in real time.
bioRxiv · Dec 6, 2023 · PreprintMicrobiology