M. Pires

Bioluminescence-Based Determination of Cytosolic Accumulation of Antibiotics in Escherichia coli

This paper introduces a luciferase-based assay to measure antibiotic permeability into the cytosol of Gram-negative bacteria and demonstrates its ability to track intracellular accumulation in real time.

Targeted Acidosis Mediated Delivery of Antigenic MHC-Binding Peptides

This study uses pH-sensitive peptides to deliver MHC-binding antigens to cancer cells, enhancing T cell activation for immunotherapy.

Non-invasive Analysis of Peptidoglycan from Living Animals

This study isolates and analyzes peptidoglycan from fecal samples of mice and humans to explore its role in host-microbiome interactions and health.

Click-Based Determination of Accumulation of Molecules in Escherichia coli

The paper develops CHAMP, a method to measure cytosolic accumulation of azide-tagged molecules in Gram-negative bacteria, enabling high-throughput screening of over 1,000 compounds.

A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice

This paper modifies BCG by depleting MurT-GatD enzymes to unmask NOD-1 ligands, enhancing TB protection in mice.

Impact of Post-Translational Modification on MHC Peptide Binding and TCR Engagement

This study investigates how post-translational modifications affect MHC peptide binding and TCR engagement, revealing their impact on antigen presentation and potential implications for autoimmune diseases and tumor neoantigens.

Measurement of Accumulation of Antibiotics to Staphylococcus aureus in Phagosomes of Live Macrophages

The paper measures antibiotic accumulation in phagosomes of live macrophages infected with Staphylococcus aureus to assess permeability differences between extracellular and intracellular environments.

Permeation Across the Mycomembrane in Live Mycobacteria

The paper develops a novel assay to measure small molecule permeation across the mycomembrane of mycobacteria, revealing variability in permeability profiles between species.

Immuno-targeting of Gram-positive Pathogens via a Cell Wall Binding Tick Antifreeze Protein

This study demonstrates the use of a tick antifreeze protein to target bacterial cell walls, enabling immune system recognition and uptake of pathogens.

Structure Activity Relationship of the Stem Peptide in Sortase A mediated Ligation from Staphylococcus aureus

The paper evaluates synthetic stem peptide analogs to assess their impact on sortase A activity in S. aureus, showing broad tolerability of substrate variations.

SaccuFlow: High-throughput Analysis Platform to Investigate Bacterial Cell Wall Interactions

SaccuFlow is a high-throughput flow cytometry assay that evaluates bacterial peptidoglycan interactions, demonstrating compatibility with Gram-positive, Gram-negative, and mycobacterial sacculi.

Packed Like Sardines - How Surface Crowdedness Impacts Accessibility to Peptidoglycan of Staphylococcus aureus

The paper introduces a live cell fluorescence assay to study molecular permeability through Staphylococcus aureus cell walls and finds that molecular flexibility and teichoic acids affect permeability.

Production of moth sex pheromones for pest control by yeast fermentation

This paper demonstrates the production of moth sex pheromones using engineered yeast fermentation, achieving commercial titres at lower cost than chemical synthesis.

Facile Synthesis and Metabolic Incorporation of m-DAP Bioisosteres Into Cell Walls of Live Bacteria

This study develops a synthetic method to create m-DAP bioisosteres for bacterial cell wall probes, demonstrating their metabolic incorporation in live bacteria.

Niche partitioning and coexistence of two spiders of the genus Peucetia (Araneae, Oxyopidae) inhabiting Trichogoniopsis adenantha plants (Asterales, Asteraceae): a population approach

This study examines niche partitioning between two sympatric spider species, showing they use different microhabitats and light conditions to reduce competition.

Analyzing ecological networks of species interactions

This paper reviews methods for analyzing ecological networks and suggests best practices for their application in community ecology.