MicrobiologybioRxiv
Click-Based Determination of Accumulation of Molecules in Escherichia coli
G. M. Ongwae, Z. Liu, S. Feng…·M. Pires
The paper develops CHAMP, a method to measure cytosolic accumulation of azide-tagged molecules in Gram-negative bacteria, enabling high-throughput screening of over 1,000 compounds.
DOI 10.1038/s41467-026-68717-5