Mapping specificity, entropy, allosteric changes and substrates in blood proteases by a high-throughput protease screen
F. Uliana, M. Vizovisek, L. Acquasaliente, R. Ciuffa, A. Fossati, F. Frommelt, M. Gstaiger, V. De Filippis, U. auf dem Keller, R. Aebersold
The paper introduces a high-throughput proteomic method to profile protease activity and substrate specificity in native conditions, achieving high throughput and accuracy.
bioRxiv · Jul 20, 2020Biochemistry