Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in E. coli
S. y. Xu, W. Yang, M. Kuska, N. Dai, L. Ettwiller
This paper tests 147 restriction enzymes on dZ-modified DNA, finds resistance in 53.1% of cases, and shows dZ incorporation in E. coli plasmids up to 44.3% with co-expression of specific genes.
bioRxiv · Oct 17, 2023 · PreprintMicrobiology