A putative rhamnogalacturonan-II CMP-β-Kdo transferase identified using CRISPR/Cas9 gene edited callus to circumvent embryo lethality.
Y. Zhang, D. Sharma, Y. Liang, N. Downs, F. Dolman, K. Thorne, I. Black, J. H. Pereira, P. D. Adams, H. Scheller, M. O'Neill, B. R. Urbanowicz, J. C. Mortimer
The paper identifies a putative rhamnogalacturonan-II CMP-β-Kdo transferase by using CRISPR/Cas9 to generate a callus mutant lacking Kdo, which disrupts RG-II crosslinking and growth.
bioRxiv · Nov 15, 2023Plant Biology