Y. Liang

Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing

This paper introduces DRAM, a method for transcriptome-wide m5C detection using deaminase and reader proteins, showing high overlap with BS-seq data and supporting low-input RNA.

Spatiotemporal transcriptomic profiling reveals the dynamic immunological landscape of alveolar echinococcosis

This study uses spatiotemporal transcriptomics to analyze immune dynamics in E. multilocularis infected mouse liver, identifying key immune cell roles and shifts in immune strategy during infection.

ALS-causing SOD1 mutations H46R and G85R form similar novel amyloid fibril structures and promote ferroptosis in cells

This study shows that two ALS-causing SOD1 mutations form similar amyloid fibrils that promote ferroptosis in neuronal cells.

Genetic variation in the species Arabidopsis thaliana reveals the existence of natural heat resilience factors for meiosis

This study identifies natural heat resilience factors in Arabidopsis thaliana that enhance meiotic stability under heat stress, with implications for crop breeding.

FLASH radiotherapy using high-energy X-rays: validation of the FLASH effect triggered by a compact single high-energy X-ray source device

This study validates the FLASH effect using a compact high-energy X-ray source in mice, showing protective effects on normal tissues but no significant tumor response differences between FLASH and conventional radiotherapy.

Clathrin-associated carriers enable recycling through a kiss-and-run mechanism

The paper identifies a novel endocytic recycling mechanism called CARP, which involves clathrin-associated carriers undergoing partial membrane fusion and release.

The functional evolution of collembolan Ubx on the regulation of abdominal appendage formation

This study investigates the functional evolution of Ubx in Folsomia candida, showing it has intermediate regulatory capabilities compared to insect and crustacean Ubx in suppressing Dll during appendage formation.

A new yeast-based bioreporter for simple, sensitive, and cost-effective detection of dioxin-like compounds

This study develops a yeast-based bioreporter, DnaSc, for sensitive and cost-effective detection of dioxin-like compounds and AhR agonists with a detection limit of 10 fM for TCDD.

Hepatic Macrophage Migration Inhibitory Factor Promotes Pancreatic Cancer Liver Metastasis in NAFLD

This study shows that hepatic MIF promotes PDAC liver metastasis in NAFLD by enhancing cancer stemness and immune suppression through the MIF-CD44 axis.

A foundation model for generalizable cancer diagnosis and survival prediction from histopathological images

This paper introduces BEPH, a lightweight foundation model for cancer diagnosis and survival prediction from histopathological images, showing improved performance over existing models with limited data.

Electroacupuncture regulates microglia activation through theSTING/NF-κB pathway to reduce pain in bone cancer pain mouse

This study shows electroacupuncture reduces bone cancer pain by inhibiting the STING/NF-κB pathway and microglia activation in the BLA.

Flat-field superresolution structured illumination microscopy with joint temporal-spatial light modulation

This paper introduces a flat-field structured illumination microscopy method to improve field uniformity and enable large-scale imaging of whole cells and pathological samples.

Diversity and ecological potentials of viral assemblages from the seamount sediments of the Northwest Pacific Ocean

The paper analyzes viral diversity in seamount sediments, identifying 78,069 vOTUs and 16 vAMGs, suggesting ecological roles in biogeochemical cycles.

High resolution cryo-EM of V-ATPase in native synaptic vesicles

The paper determines the high-resolution structure of V-ATPase in native synaptic vesicles using cryo-EM, revealing interactions with cholesterol and synaptophysin.

Protein disulfide isomerase disassembles stress granules and blocks cytoplasmic aggregation of TDP-43 in ALS

This study shows that wild-type PDI disassembles stress granules and prevents TDP-43 mislocalization and aggregation in ALS, while abnormal PDI leads to amyloid fibrils and neuronal death.

Boosting the Sensitivity of Quantitative Single-Cell Proteomics with Activated Ion-Tandem Mass Tags (AI-TMT)

This paper introduces AI-TMT, a method enhancing single-cell proteomics sensitivity by improving reporter ion generation, resulting in higher signal and faster analysis.

Lack of the IFN-γ signal leads to lethal Orientia tsutsugamushi infection in mice with skin eschar lesions

This study shows that lack of IFN-γ signaling leads to lethal Orientia tsutsugamushi infection in mice with skin eschar lesions, highlighting its critical role in immune defense.

CD8+ T cell response promotes viral clearance and reduces chances of severe testicular damage in mouse models of long-term Zika virus infection of the testes.

This study shows CD8+ T cells reduce severe testicular damage in mice with long-term Zika virus infection by promoting viral clearance.

Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation

This study identifies USP8's role in endosome maturation by mediating Rab5-to-Rab7 switch through recruitment by Rabex5 and promotion of SAND-1/Mon1 recruitment.

Evolution of plant cell-type-specific cis-regulatory elements

The paper constructs a single-cell chromatin accessibility atlas in rice and compares it across grass species to study the evolution of cell-type-specific cis-regulatory elements.

Spatial characterization of interface dermatitis in cutaneous lupus reveals novel chemokine ligand-receptor pairs that drive disease

This study identifies novel chemokine ligand-receptor pairs involved in immune cell recruitment in cutaneous lupus by combining spatial transcriptomics, proteomics, and immunological assays.

Association of poultry vaccination with the interspecies transmission and molecular evolution of H5 subtype avian influenza virus

This study examines how poultry vaccination affects H5 avian influenza virus transmission and evolution, finding increased nonsynonymous divergence in vaccinated poultry lineages.

Parallel Molecular Data Storage by Printing Epigenetic Bits on DNA

This paper introduces a DNA-based data storage method using epigenetic modifications, achieving scalable and parallel data writing and retrieval.

Effect of mechanical stretching stimulation on maturation of human iPS cell-derived cardiomyocytes co-cultured with human gingival fibroblasts

This study shows that mechanical stretching improves the maturation and differentiation of human iPS cell-derived cardiomyocytes co-cultured with fibroblasts.

Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem function

This paper introduces FISH-scRACS-Seq, a method to identify and sequence single cells with specific metabolic functions, revealing a new cyclohexane-degrading enzyme system in marine bacteria.

Host signature as major driver of root and rhizosphere core microbiomes that differently affect plant functional traits

The paper identifies plant traits as the main driver of core microbiomes in different root compartments and shows their distinct impacts on plant metal accumulation and growth.

A putative rhamnogalacturonan-II CMP-β-Kdo transferase identified using CRISPR/Cas9 gene edited callus to circumvent embryo lethality.

The paper identifies a putative rhamnogalacturonan-II CMP-β-Kdo transferase by using CRISPR/Cas9 to generate a callus mutant lacking Kdo, which disrupts RG-II crosslinking and growth.

Glomerular immune injury promotes anti-tumor activity

This study shows that glomerular immune injury increases anti-tumor activity by altering bone marrow transcription in mice.

In silico MS/MS prediction for peptidoglycan profiling uncovers novel anti-inflammatory peptidoglycan fragments of the gut microbiota

This paper develops a computational tool to predict peptidoglycan MS/MS spectra, enabling automated profiling of gut microbiota-derived PGNs and identifying anti-inflammatory anhydro-PGNs in Bifidobacterium.

Targeting the Hippo pathway in cancers via ubiquitination dependent TEAD degradation

This study identifies RNF146 as an E3 ubiquitin ligase that degrades TEAD proteins via the proteasome, showing that pharmacological induction of TEAD ubiquitination can suppress YAP/TAZ-driven cancer growth.