Split-luciferin Assay for Real-time Measurement of Cytosolic Molecular Accumulation in Live Mycobacteria
Rachita Dash, Andrew Spira, Irene Lepori, Sarah E. Newkirk, Sobika Bhandari, Noah Lucas, Mahendra D. Chordia, M. Sloan Siegrist, Marcos M. Pires
This paper introduces D-CLAP, a split-luciferin assay to measure real-time cytosolic accumulation of compounds in live mycobacteria, showing that modifying carboxylates increases accumulation and antimycobacterial activity.
bioRxiv · Nov 2, 2025 · PreprintMicrobiology