MicrobiologyAntimicrobial Agents and ChemotherapyPeer-reviewed
Improving Nitrofurantoin Resistance Prediction in Escherichia coli from Whole Genome Sequence by Integrating NfsA/B Enzyme Assays
Punyawee Dulyayangkul, Jordan E. Sealey, Winnie W. Y. Lee…·Matthew B. Avison
The paper identifies specific amino acid substitutions in NfsA and NfsB that reduce enzyme activity and cause nitrofurantoin resistance in E. coli, but finds that WGS alone cannot reliably predict resistance due to functional silent mutations and downregulated gene expression.
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