Structural and mechanistic insight into spectral tuning in flavin-binding fluorescent proteins
K. Roellen, J. Granzin, A. Remeeva, M. D. Davari, T. Gensch, V. V. Nazarenko, K. Kovalev, A. Bogorodskiy, V. Borshchevskiy, S. Hemmer, U. Schwaneberg, V. Gordeliy, K.-E. Jaeger, R. Batra-Safferling, I. Gushchin, U. Krauss
The paper identifies structural and mechanistic causes of spectral tuning in flavin-binding fluorescent proteins through mutagenesis, X-ray crystallography, and computational methods, enabling two-color microscopy.
bioRxiv · Jan 8, 2021Biophysics