K.-E. Jaeger

Molecular Mechanisms Underlying Medium-Chain Free Fatty Acid-regulated Activity of the Phospholipase PlaF from P. aeruginosa

This study investigates how medium-chain free fatty acids regulate PlaF activity through molecular dynamics simulations and free energy computations, revealing mechanisms of dimerization and activation.

The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa

This study shows that Skp prevents misfolding of lipase A in Pseudomonas aeruginosa and facilitates its activation by LipH, with two binding modes identified.

Substrate access mechanism in a novel membrane-bound phospholipase A of Pseudomonas aeruginosa concordant with specificity and regioselectivity

This study identifies the substrate access pathway and active site tunnel of PlaF, revealing how its structure influences specificity and regioselectivity in phospholipase A1 activity.

Promiscuous esterases counterintuitively are less flexible than specific ones

The paper shows that promiscuous esterases are less flexible and more thermostable than specific ones, challenging the common assumption about enzyme flexibility and promiscuity.

Structural and mechanistic insight into spectral tuning in flavin-binding fluorescent proteins

The paper identifies structural and mechanistic causes of spectral tuning in flavin-binding fluorescent proteins through mutagenesis, X-ray crystallography, and computational methods, enabling two-color microscopy.

Ustilago maydis serves as a novel production host for the synthesis of plant and fungal sesquiterpenoids

This paper demonstrates that Ustilago maydis can produce fungal and plant sesquiterpenoids, achieving higher titres for fungal compounds than plant ones.