R. Tuma

Unique and Common Agonists Activate the Insect Juvenile Hormone Receptor and the Human AHR

The paper identifies common and unique agonists for the insect JHR and human AHR receptors, showing overlap in their ligand repertoires and structural interactions.

Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation by the bacterial Sec machinery

This study uses single-molecule fluorescence to show that SecYEG dynamically couples with SecA through energy landscape steering, enhancing protein translocation efficiency.

Mechanistic insight into the ATP hydrolysis cycle of tick-borne encephalitis virus helicase

The paper investigates the ATP hydrolysis mechanism of tick-borne encephalitis virus NS3H helicase, showing RNA specificity and structural insights into its activity.

Structural basis of rotavirus RNA chaperone displacement and RNA annealing

This study identifies how the rotavirus NSP2 protein regulates RNA chaperone activity through structural changes that promote RNA annealing and release.

Inter-domain dynamics in the chaperone SurA and multi-site binding to its unfolded outer membrane protein clients

This study investigates the conformational dynamics of SurA and its interaction with unfolded OMP clients using multiple biophysical techniques.

Stability of local secondary structure determines selectivity of viral RNA chaperones

This study compares the ability of viral RNA chaperones NSP2 and σNS to promote inter-segment RNA-RNA interactions, finding that NSP2's high affinity for structured RNAs and efficient helix disruption enable successful inter-segment association, while σNS lacks this capacity.

Dynamic action of the Sec machinery during initiation, protein translocation and termination revealed by single molecule fluorescence

This study uses single molecule FRET to analyze Sec machinery dynamics during protein translocation, revealing initiation, transport, and termination phases with specific roles for SecA and ATP hydrolysis.