Preparation of next-generation DNA sequencing libraries from ultra-low amounts of input DNA: Application to single-molecule, real-time (SMRT) sequencing on the Pacific Biosciences RS II.
Castle Raley, David Munroe, Kristie Jones, Yu-Chih Tsai, Yan Guo, Bao Tran, Sujatha Gowda, Jennifer L. Troyer, Daniel R Soppet, Claudia Stewart, Robert Stephens, Jack Chen, TF Skelly, Cheryl Heiner, Jonas Korlach, Dwight Nissley
The paper develops an amplification-free method for preparing DNA sequencing libraries from very low DNA input for PacBio SMRT sequencing, achieving comparable yields with 1000-fold less starting material.
bioRxiv · Mar 25, 2014 · PreprintGenomics