Emergence of NADP+-reducing enzymes in Escherichia coli central metabolism via adaptive evolution
M. Bouzon, V. Döring, I. Dubois, A. Berger, G. M. M. Stoffel, L. Calzadiaz Ramirez, S. Meyer, M. Foure, D. Roche, A. Perret, T. J. Erb, A. Bar-Even, S. N. Lindner
This study shows that adaptive evolution in E. coli can change cofactor specificity of enzymes, enabling NADPH regeneration without external sources, but is limited by thermodynamic constraints.
bioRxiv · Feb 8, 2021 · PreprintMicrobiology