Systems BiologybioRxivPreprint
Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library
Aida Tafrishi, Varun Trivedi, Zenan Xing…·Ian R. Wheeldon
The paper develops a high-activity CRISPR-Cas9 library for Komagataella phaffii and uses it to identify essential genes related to protein production, secretion, and glycosylation.
DOI 10.1101/2024.02.08.579509