Chemical control of 2'-hydroxyl-dependent Cas9 target engagement enables CRISPR RNA ribose replacement
Adrian A. Pater, Halle M. Barber, Sruthi Sudhakar…·Keith T. Gagnon
This study identifies the role of 2'-hydroxyl groups in crRNA for Cas9 DNA binding and develops chemically stabilized crRNAs with enhanced nuclease resistance and editing efficiency.