Measuring cis-regulatory energetics in living cells using allelic manifolds
T. Forcier, A. Ayaz, M. S. Gill, D. Jones, R. Phillips, J. B. Kinney
The paper introduces a method to measure the Gibbs free energy of TF-DNA and TF-TF interactions in living cells using allelic manifolds and reporter assays, achieving precision of ~0.1 kcal/mol.
bioRxiv · Nov 19, 2018Biophysics