Sequential, Chromosome-Specific Glutamine Synthetase Double Knockout with Cas-CLOVER Establishes Enhanced CHO Platforms for Cell Line Development
Cintia Elisabeth Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark C. McHargue, Corey O. Brizzee, Jack Crawford
This study uses Cas-CLOVER to create a double glutamine synthetase knockout CHO cell line with enhanced productivity for biopharmaceutical production.
Biotechnology Progress · Feb 22, 2026Peer-reviewedBioengineering