V. A. Risso

Ancestral versus Modern Substrate Scope in Family-1 Glycosidases

This study compares the substrate scope of a modern and ancestral family-1 glycosidase, finding the ancestral enzyme more efficient with large aglycon-containing flavonoid glycosides.

Efficient base-catalysed Kemp elimination in an engineered ancestral enzyme

This study engineers an ancestral β-lactamase to efficiently catalyze Kemp elimination, achieving catalytic parameters comparable to modern enzymes.

Combining ancestral reconstruction with folding-landscape simulations to engineer heterologous protein expression

This study engineers a symbiont thioredoxin for efficient heterologous expression by resurrecting ancestral proteins and optimizing folding rates.

Evidence for a role of phenotypic mutations in virus adaptation

This study shows that virus adaptation can occur through phenotypic mutations rather than genetic mutations in the viral DNA polymerase.

Non-conservation of folding rates in the thioredoxin family reveals degradation of ancestral unassisted-folding

The paper analyzes folding rates of thioredoxins across species and finds that modern variants fold slower than ancestral ones, suggesting degradation of unassisted folding.