V. Walbot

FX-Cell: Quantitative cell release from fixed plant tissues for single-cell genomics

FX-Cell improves cell release from fixed plant tissues for scRNA-seq, yielding more cells with no bias and enabling cell type identification.

24-nt phasiRNAs move from tapetal to meiotic cells in maize anthers

The paper shows 24-nt phasiRNAs move from tapetal cells to meiocytes and other somatic cells in maize anthers.

Gametophyte genome activation occurs at pollen mitosis I in maize

This study identifies the timing of haploid genome activation in maize pollen, showing a shift to monoallelic expression at pollen mitosis I and evidence of purifying selection during the haploid phase.

Environment-conditioned male fertility of HD-ZIP IV transcription factor mutant ocl4: impact on 21-nt phasiRNA accumulation in pre-meiotic maize anthers

The paper investigates how the HD-ZIP IV transcription factor mutant ocl4 affects 21-nt phasiRNA accumulation in maize anthers under different environmental conditions, finding that these phasiRNAs buffer development under stress.

Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize

This study shows that Dicer-like 5 deficiency in maize leads to temperature-sensitive male sterility and defects in anther development.

Defining the developmental program leading to meiosis in maize

This study uses single-cell RNA-seq to define the developmental program leading to meiosis in maize, revealing a multi-step pathway and transcriptional reorganization before meiotic prophase.