Synthetic BiologyNature MethodsPeer-reviewed
Burden-driven feedback control of gene expression
Francesca Ceroni, Alice Boo, Simone Furini…·Tom Ellis
The paper identifies heat-shock promoters that activate rapidly in response to synthetic gene expression in E. coli and uses them to build a CRISPR/dCas9-based feedback system that improves protein yield by adjusting synthetic construct expression based on cellular burden.
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