BiochemistrybioRxivPreprint
Employing neutron-encoded monoUbs to study E2/E3 ligase activity and selectivity for assembling Ub chains
Bianca D. M. van Tol, Bjorn R. van Doodewaerd, Guinevere S. M. Lageveen‐Kammeijer…·Paul P. Geurink
The paper develops a mass spectrometry-based assay to screen E2/E3 ligase pairs for their ability to form ubiquitin chains and analyze chain types.