Mutations in the tomato gibberellin receptors suppresses xylem proliferation and reduces water loss under water-deficit conditions

Abstract

Low gibberellin (GA) activity in tomato (Solanum lycopersicum) inhibits leaf expansion and reduces stomatal conductance. These lead to lower transpiration and improve water status under transient drought conditions. Tomato has three GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptors with overlapping activities and high redundancy. We have tested whether mutation in a single GID1 reduces transpiration without affecting growth and productivity. CRISPR-Cas9 gid1 mutants were able to maintain higher leaf water content under water-deficit conditions. Moreover, while gid1a exhibited normal growth, it showed reduced whole plant transpiration and better recovery from dehydration. Mutation in GID1a inhibited xylem vessels proliferation that led to lower hydraulic conductance. In stronger GA mutants, we also found reduced xylem vessel expansion. These results suggest that low GA activity affects transpiration by multiple mechanisms; it reduces leaf area, promotes stomatal closure and reduces xylem proliferation and expansion and as a result, xylem hydraulic conductance. We further examined if gid1a perform better than the control M82 in the field. Under these conditions, the high redundancy of GID1s was lost and gid1a plants were semi-dwarf, but their productivity was not affected. Although gid1a did not perform better under drought conditions in the field, it exhibited higher harvest index. HighlightThe loss of the tomato gibberellin receptors GID1s reduced xylem proliferation and xylem hydraulic conductance. These contribute to the effect of low gibberellin activity on water loss under water-deficit condition.