Plasma Contains Ultra-short Single-stranded DNA in Addition to Nucleosomal cfDNA
Jordan C. ChengiD, Marco MorselliiD, Wei-Lun HuangiD, You Jeong HeoiD, Thalyta Pinheiro-FerreiraiD, Feng LiiD, Wei FangiD, David ChiaiD, Hua Jun, Kenneth D. ColeiD, Wu‐Chou SuiD, Matteo A. PellegriniiD, David T. Wong
Abstract
Plasma cell-free DNA is a widely used biomarker for diagnostic screening. We introduce uscfDNA-seq, a single-stranded cell-free DNA NGS pipeline, which bypasses previous limitations to reveal a novel population of ultrashort single-stranded cell-free DNA in plasma with a modal size of 50nt. This species of cfDNA aligns predominantly to the nuclear genome and could potentially be used for novel biomarker discovery.
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