bioRxivMolecular BiologyPreprint
Transcriptional activity of TRF2 is telomere length dependent
Ananda Kishore Mukherjee, Shalu Sharma, Parashar Dhapola, Dhurjhoti Saha, Tabish Hussain, Sumitabho Deb Roy, Gunjan Purohit, Anirban Kar, Ankita Singh, Suman Sengupta, Vivek Srivastava, Manish Kumar, Sagar Sengupta, Shantanu Chowdhury
Abstract
TRF2 is a telomere repeat binding factor crucial for telomere maintenance and genome stability. An emerging non-conventional role of TRF2 is as a transcriptional regulator through extra-telomeric bindings. Herein we report that increase in telomere length leads to sequestration of TRF2 at the telomeres leading to reduced extra-telomeric TRF2 occupancy genome wide. Decrease in TRF2 occupancy was found on multiple gene promoters in cells with elongated telomeres, including the cell cycle regulator kinase-p21. We found that TRF2 is a transcriptional repressor of p21, and, interestingly, TRF2-mediated regulatory control of p21 is telomere length dependent.
- organism: human
- research focus: differential expression
- research focus: gene regulatory network inference
- approach: statistical model
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