Sylites: Multipurpose markers for the visualization of inhibitory synapses

Vladimir Khayenko; Clemens Schulte; Sara L. Reis; Orly Avraham; Cataldo Schietroma; Rafael Worschech; Noah Frieder Nordblom; Sonja Kachler; Carmen Villmann; Katrin Gertrud Heinze; Andreas Schlösser; Ora Schueler‐Furman; Philip Tovote; Christian G. Specht; Hans Michael Maric

doi:10.1101/2021.05.20.444651

NeurosciencebioRxivMay 20, 2021

Preprint

Sylites: Multipurpose markers for the visualization of inhibitory synapses

Vladimir Khayenko iD, Clemens Schulte iD, Sara L. Reis iD, Orly Avraham iD, Cataldo Schietroma iD, Rafael Worschech, Noah Frieder Nordblom iD, Sonja Kachler, Carmen Villmann iD, Katrin Gertrud Heinze iD, Andreas Schlösser iD, Ora Schueler‐Furman iD, Philip Tovote iD, Christian G. Specht iD, Hans Michael Maric iD

Abstract

We introduce Sylites - small and versatile fluorogenic affinity probes for high-contrast visualization of inhibitory synapses. Having stoichiometric labeling and exceptional selectivity for neuronal gephyrin, a hallmark protein of the inhibitory post-synapse, Sylites enable superior synapse staining compared with antibodies. Combined with super-resolution microscopy, Sylites allow precise nanoscopic measurements of the synapse. In brain tissue, Sylites reveal the three-dimensional distribution of inhibitory synapses within just an hour.

PDF DOI