Analysis of polyurethane/gelatin complex hydrogel system for protein imprinting

Polyurethane served as the carrier in the synthesis of a hydrogel system, incorporating membrane protein as the template, N-Vinylformamide as the monomer, and 1,4-Butanediol diglycidyl ether as the crosslinker, along with gelatin, initiated by ultraviolet radiation. This resulted in the formation of the hydrogel PUNVF-Gelatin. The study investigated the influence of varying monomer concentration, crosslinker concentration, and gelatin concentration on both the adsorption capacity for membrane protein and the imprinting efficiency. Findings revealed that optimal conditions for achieving the maximum adsorption capacity occurred when the monomer mass fraction was 5%, the crosslinker mass fraction was 3%, and the gelatin mass fraction was 0.6%.