Protein and DNA Conformational Changes Contribute to Specificity of Cre Recombinase

Abstract

Cre, a conservative site-specific tyrosine recombinase, is a powerful gene editing tool in the laboratory. Expanded applications in human health are hindered by lack of understanding of the mechanism by which Cre selectively binds and recombines its cognate loxP sequences. This knowledge is essential for retargeting the enzyme to new sites and for mitigating effects of off-target recombination. Prior studies have suggested that in addition to a few base-specific contacts to cognate loxP DNA, the enzymes specificity is enhanced by (1) autoinhibition involving a conformational change in the proteins C-terminal helix, and (2) indirect readout via binding-coupled conformational changes in the target DNA. We used isothermal titration calorimetry (ITC), circular dichroism (CD) and heteronuclear NMR spectroscopy to investigate DNA site recognition by wild-type Cre and a deletion mutant lacking the C-terminal helix. ITC of Cre and a C-terminal deletion variant against cognate and non-cognate DNA recombinase binding elements (RBEs) reveal that the C-terminus reduces DNA binding affinity by six-fold towards cognate DNA. Additionally, ITC revealed highly unfavorable binding enthalpy, which when combined with evidence from CD and NMR of structural differences between cognate and non-cognate complexes support a model in which binding-coupled DNA bending provides a unique structure-thermodynamic signature of cognate complexes. Together, these findings advance our understanding of site-recognition by Cre recombinase. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=126 SRC="FIGDIR/small/627928v1_ufig1.gif" ALT="Figure 1"> View larger version (24K): org.highwire.dtl.DTLVardef@19fb34dorg.highwire.dtl.DTLVardef@1b4b025org.highwire.dtl.DTLVardef@1988254org.highwire.dtl.DTLVardef@1945cb1_HPS_FORMAT_FIGEXP M_FIG C_FIG