EpiMethylTag simultaneously detects ATAC-seq or ChIP-seq signals with DNA methylation

Priscillia Lhoumaud; Gunjan Sethia; Franco Izzo; Theodore Sakellaropoulos; Valentina Snetkova; Simon E. Vidal; Sana Badri; MacIntosh Cornwell; Dafne Campigli Di Giammartino; Kyu‐Tae Kim; Effie Apostolou; Matthias Stadtfeld; Dan Avi Landau; Jane A. Skok

doi:10.1186/s13059-019-1853-6

GenomicsGenome biologyNov 21, 2019

Peer-reviewed journal article

EpiMethylTag simultaneously detects ATAC-seq or ChIP-seq signals with DNA methylation

Priscillia Lhoumaud iD, Gunjan Sethia iD, Franco Izzo iD, Theodore Sakellaropoulos iD, Valentina Snetkova iD, Simon E. Vidal, Sana Badri, MacIntosh Cornwell iD, Dafne Campigli Di Giammartino iD, Kyu‐Tae Kim, Effie Apostolou iD, Matthias Stadtfeld iD, Dan Avi Landau iD, Jane A. Skok iD

Abstract

Activation of regulatory elements is thought to be inversely correlated with DNA methylation levels. However, it is difficult to determine whether DNA methylation is compatible with chromatin accessibility or transcription factor (TF) binding if assays are performed separately. We developed a low input, low sequencing depth method, EpiMethylTag that combines ATAC-seq or ChIP-seq (M-ATAC or M-ChIP) with bisulfite conversion, to simultaneously examine accessibility/TF binding and methylation on the same DNA.

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