A defined 2D system for generating and expanding human basal radial glia from iPSCs

Annasara Artioli; Matteo Gasparotto; A.C. Rossetti; Yannick Hass; Anne Hoffrichter; Ryszard Wimmer; Fabio Marsoner; Raquel Perez Fernandez; Catello Guida; Philipp Koch; Alexandre D. Baffet; Ammar Jabali; Julia Ladewig

doi:10.64898/2026.04.10.714524

NeurosciencebioRxivApr 14, 2026

Preprint

A defined 2D system for generating and expanding human basal radial glia from iPSCs

Annasara Artioli, Matteo Gasparotto iD, A.C. Rossetti iD, Yannick Hass, Anne Hoffrichter iD, Ryszard Wimmer iD, Fabio Marsoner, Raquel Perez Fernandez, Catello Guida, Philipp Koch iD, Alexandre D. Baffet iD, Ammar Jabali iD, Julia Ladewig iD

Abstract

Basal radial glia (bRG) drive human cortical expansion but remain underrepresented in vitro. We established a defined and expandable 2D system for efficient generation of human bRG from iPSCs. These cells recapitulate canonical molecular signatures, hallmark somal translocation behaviours, intrinsic differentiation potential, and integration into organoid tissue. Network-based analyses identified PAK2 as a regulator of mitotic somal translocation, illustrating the systems utility for mechanistic interrogation of bRG biology.

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