From Field to FASTA: Onsite DNA Barcoding the Mushrooms of the 2024 NAMA Foray

Harte Singer; Stephen Douglas Russell; Andrew W. Wilson; Mandie Quark; April Coulon; Andrew MacPherson; Kevin Young; Toni Doucette; Scott Ostuni; Maria Marlin; E. Dods; Matthew Gilbert Koons; Joshua Birkebak

doi:10.1101/2025.07.16.664919

GeneticsbioRxivJul 16, 2025

Preprint

From Field to FASTA: Onsite DNA Barcoding the Mushrooms of the 2024 NAMA Foray

Harte Singer iD, Stephen Douglas Russell iD, Andrew W. Wilson iD, Mandie Quark iD, April Coulon, Andrew MacPherson, Kevin Young, Toni Doucette, Scott Ostuni iD, Maria Marlin iD, E. Dods, Matthew Gilbert Koons, Joshua Birkebak iD

Abstract

Mushroom forays present unparalleled opportunities to record macrofungal biodiversity in a place and time. The 2024 NAMA annual foray at the Cispus Center near Randle, Washington was the first mass barcoding activity performed by the NAMA DNA Sequencing Committee (DNAMA). The workflows and methods utilized are presented along with planned improvements. Ultimately, 803 ITS barcodes were generated (83.7% of those attempted) for 513 unique taxa belonging to 209 Genera. Of these, 18 novel putative species-level operational taxonomic units (OTUs) were discovered as well as 28 new distribution reports at state level or above. Methods and suggestions for replicating and improving mass barcoding at forays are discussed.

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