Reconstruction of genome-scale metabolic model for Hansenula polymorpha using RAVEN toolbox

Genome scale metabolic models (GEMs) provide a useful framework for modeling the metabolism of microorganisms. While the applications of GEMs are wide and far reaching, the reconstruction and continuous curation of such models can be perceived as a tedious and time consuming task. Using the RAVEN toolbox, this protocol practically demonstrates how researchers can create their own GEMs using a homology based approach. To provide a complete example, we reconstruct a draft GEM for the industrially relevant yeast Hansenula polymorpha.