PLoS ONEGenomicsPeer-reviewed journal article
Single Molecule Sequencing Of M13 Virus Genome Without Amplification
Luyang ZhaoiD, Liwei DengiD, Gailing LiiD, Huan Jin, Jinsen CaiiD, Huan ShangiD, Yan LiiD, Haomin Wu, Weibin XuiD, Lidong ZengiD, Renli ZhangiD, Huan ZhaoiD, Ping Chun WuiD, Zhiliang Zhou, Jiao ZhengiD, Pierre Ezanno, Andrew X. YangiD, Qin Yan, Michael W. DeemiD, Jiankui HeiD
Abstract
Third generation sequencing is a direct measurement of DNA/RNA sequences at the single molecule level without amplification. In this study, we report sequencing of the genome of the M13 virus by a new single molecule sequencing platform. Our platform detects single molecule fluorescence by the total internal reflection microscope technique, with sequencing-by-synthesis chemistry. We sequenced the genome of M13 to a depth of 316x and 100% coverage. The consensus sequence accuracy is 100%. We demonstrated that single molecule sequencing has no significant GC bias.
- Citations
- 16
- FWCI
- 0.89
- Field percentile
- top 28%
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